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Production of artificial vesicle, a bio-mimetic object to study malaria parasite egress

Cyrille Claudet

Jeudi 2 juillet 2015

à 11h en salle C. Brot

I will described a method to produce large quantities of lipids bilayer vesicle. This method was called c-DICE for « Continuous Droplet Interface Crossing Encapsulation ». It consists of dripping aqueous droplets at the center of a rotating chamber filled with an oil phase and an aqueous phase. The rotation of the chamber exerts a centrifugal force on the droplets that are forced to travel through the oil. A first layer of lipids builds up during the travel through the oil phase by adsorption of lipids dispersed in the oil phase. The second layer of lipids builds up while crossing the lipid- covered interface by a zipping process between the adsorbed layer on the droplet and the lipid layer adsorbed at the oil water interface. There are many advantages of this simple process over previously existing methods such as low polydispersity and high yield and also time saving. In fact, an original technics have been used to disperse lipid in the oil phase, based on an “ouzo effect”. I will then introduce some experiments we have performed with these vesicle, to study some mechanical properties of the membrane of an infected red blood cell by the malaria parasite.